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Nexcelom Bioscience cellometer auto 2000
Cellometer Auto 2000, supplied by Nexcelom Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cellometer+auto+2000/cellometer+auto+2000/pmc11735100-238-18-21
Average 90 stars, based on 1 article reviews
cellometer auto 2000 - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Transduction:

Article Title: CD28 Costimulation Augments CAR Signaling in NK Cells via the LCK/CD3ζ/ZAP70 Signaling Axis.
Article Snippet: .. An equal number of 557 NK cells from each condition were expanded on day 2 post transduction and cell counts were 558 recorded using ViaStainTM AOPI Staining Solution (Nexcelom) and cellometer Auto 2000 559 (Nexcelom) instrument over time at the time of media change to monitor the proliferative 560 capacity of NK cells. ..

Staining:

Article Title: CD28 Costimulation Augments CAR Signaling in NK Cells via the LCK/CD3ζ/ZAP70 Signaling Axis.
Article Snippet: .. An equal number of 557 NK cells from each condition were expanded on day 2 post transduction and cell counts were 558 recorded using ViaStainTM AOPI Staining Solution (Nexcelom) and cellometer Auto 2000 559 (Nexcelom) instrument over time at the time of media change to monitor the proliferative 560 capacity of NK cells. ..

Article Title: Combinatorial effector targeting (COMET) for transcriptional modulation and locus-specific biochemistry
Article Snippet: .. All iPSC counting was performed on a Nexcelom Cellometer Auto 2000 using acridine orange/propidium iodide (AOPI) stain (Nexcelom). (T0) Approximately 12.8×10 6 cells were plated on cultrex-coated T175s, in 20mL mTeSR Plus and 1μM THZ, so that the cells cover the surface area of the plate in low volume (as previously described ). .. Eight days post infection and three days post thaw, cells were harvested, resuspended in freshly prepared FACS buffer to a final concentration of 10e6 cells/ml, and stained with anti-CD55-APC (Biolegend) antibody for 25 minutes on ice.

Article Title: Postischemic inactivation of HIF prolyl hydroxylases in endothelium promotes maladaptive kidney repair by inducing glycolysis
Article Snippet: .. Cell number and viability were analyzed using the Nexcelom Cellometer Auto 2000 with the AOPI fluorescent staining method. ..

Cell Culture:

Article Title: IL-27 as a novel biomarker for pruritus in nodular prurigo and bullous pemphigoid
Article Snippet: PBMCs were isolated using Lymphocyte Separation Medium (Corning, Manassas, VA) via centrifugation. .. Cell counts were performed using the Cellometer Auto 2000 (Nexcelom, Lawrence, MA) and cells were cultured at 2 × 10 6 cells/ml. .. RNA was extracted from PBMCs using TRIzol (Invitrogen, Carlsbad, CA).

Single Cell:

Article Title: NOTCH1 drives tumor plasticity and metastasis in hepatocellular carcinoma
Article Snippet: .. To this end, single-cell suspensions were counted (Nexcelom Cellometer Auto 2000) and loaded on one lane of the 10x Genomics NextGem 5’v2.0 assay per the manufacturer’s protocol, with a targeted cell recovery of 10,000 cells per lane. .. Per the 10x Genomics protocol ( https://assets.ctfassets.net/an68im79xiti/57JaTECQNBPSpyDz8oucdi/ced6aa8eaf73d6ee18dea8fdbd945faa/CG000331_Chromium_Next_GEM_Single_Cell_5-v2_UserGuide_RevD.pdf ), gene expression (5’Gex) and TCR libraries were generated then quantified via Agilent Technologies 4200 Tapestation and KAPA library quantification kit (Roche).

Cell Recovery:

Article Title: NOTCH1 drives tumor plasticity and metastasis in hepatocellular carcinoma
Article Snippet: .. To this end, single-cell suspensions were counted (Nexcelom Cellometer Auto 2000) and loaded on one lane of the 10x Genomics NextGem 5’v2.0 assay per the manufacturer’s protocol, with a targeted cell recovery of 10,000 cells per lane. .. Per the 10x Genomics protocol ( https://assets.ctfassets.net/an68im79xiti/57JaTECQNBPSpyDz8oucdi/ced6aa8eaf73d6ee18dea8fdbd945faa/CG000331_Chromium_Next_GEM_Single_Cell_5-v2_UserGuide_RevD.pdf ), gene expression (5’Gex) and TCR libraries were generated then quantified via Agilent Technologies 4200 Tapestation and KAPA library quantification kit (Roche).

Suspension:

Article Title: Systemic and local chronic inflammation and hormone disposition promote a tumor-permissive environment for breast cancer in older women
Article Snippet: Snap frozen tissues are dissociated with the 10X Genomics Nuclei Isolation Kit. .. After dissociation, nuclei suspension concentration and nuclei integrity are assessed using AOPI on the Nexcelom Cellometer Auto 2000. .. Libraries are then prepared according to the Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (Dual Index) protocol using the 10X Genomics Chromium iX Instrument.

Concentration Assay:

Article Title: Systemic and local chronic inflammation and hormone disposition promote a tumor-permissive environment for breast cancer in older women
Article Snippet: Snap frozen tissues are dissociated with the 10X Genomics Nuclei Isolation Kit. .. After dissociation, nuclei suspension concentration and nuclei integrity are assessed using AOPI on the Nexcelom Cellometer Auto 2000. .. Libraries are then prepared according to the Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (Dual Index) protocol using the 10X Genomics Chromium iX Instrument.



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